Coding

Part:BBa_K2223003:Design

Designed by: Thomas Sousa   Group: iGEM17_UCSC   (2017-11-01)


bluB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 680
    Illegal NheI site found at 684
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had to be codon optimized for use in Synechococcus elongatus PCC 7942. Homologous arms were added later for recombination into the genome of S. elongatus PCC 7942.


Source

Sinorhizobium meliloti

References