Coding
Part:BBa_K2223003:Design
Designed by: Thomas Sousa Group: iGEM17_UCSC (2017-11-01)
bluB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 680
Illegal NheI site found at 684 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 648
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence had to be codon optimized for use in Synechococcus elongatus PCC 7942. Homologous arms were added later for recombination into the genome of S. elongatus PCC 7942.
Source
Sinorhizobium meliloti